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h3 genome wide grna library  (Addgene inc)


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    Structured Review

    Addgene inc h3 genome wide grna library
    ( A ) Cartoon schematic of the genome-wide CRISPR/Cas9-knockout (KO) screen; (step 1) T47D cells are infected with the <t>H3</t> lentiviral library of gRNAs, (step 2) infected cells are selected using puromycin, (step 3) cells are collected for untreated baseline (Day 0), or treated with DMSO or 1 nM imlunestrant, (step 4) after treatment for 14 or 31 days cells are collected, gRNAs amplified, and sequenced, followed by (step 5) <t>gRNA</t> analysis using MAGeCK algorithm. ( B ) MAGeCK β score and ranking results for gRNAs enriched or depleted after treatment with DMSO versus Day 0 for 14 days or ( C ) 31 days. ( D ) MAGeCK β score and ranking results for gRNAs enriched or depleted after treatment with imlunestrant versus Day 0 for 14 days or ( E ) 31 days. Scatter plot with each dot represents a gene in the genome-wide gRNA library, red are enriched after treatment (β ≥ +1), blue are depleted after treatment (β ≤ –1), black are insignificant after treatment, genes in the ER pathway are orange, the CDK pathway are purple, and the MTORC1 pathway are green. ( F ) β scores for individual genes depleted after imlunestrant treatment in the ER pathway, ( G ) the CDK pathway, or ( H ) the MTORC1 pathway.
    H3 Genome Wide Grna Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/h3+genome+wide+grna+library/pmc12226049-247-7-12?v=Addgene+inc
    Average 93 stars, based on 51 article reviews
    h3 genome wide grna library - by Bioz Stars, 2026-07
    93/100 stars

    Images

    1) Product Images from "Imlunestrant a next-generation oral SERD overcomes ESR1 mutant resistance in estrogen receptor–positive breast cancer"

    Article Title: Imlunestrant a next-generation oral SERD overcomes ESR1 mutant resistance in estrogen receptor–positive breast cancer

    Journal: JCI Insight

    doi: 10.1172/jci.insight.188051

    ( A ) Cartoon schematic of the genome-wide CRISPR/Cas9-knockout (KO) screen; (step 1) T47D cells are infected with the H3 lentiviral library of gRNAs, (step 2) infected cells are selected using puromycin, (step 3) cells are collected for untreated baseline (Day 0), or treated with DMSO or 1 nM imlunestrant, (step 4) after treatment for 14 or 31 days cells are collected, gRNAs amplified, and sequenced, followed by (step 5) gRNA analysis using MAGeCK algorithm. ( B ) MAGeCK β score and ranking results for gRNAs enriched or depleted after treatment with DMSO versus Day 0 for 14 days or ( C ) 31 days. ( D ) MAGeCK β score and ranking results for gRNAs enriched or depleted after treatment with imlunestrant versus Day 0 for 14 days or ( E ) 31 days. Scatter plot with each dot represents a gene in the genome-wide gRNA library, red are enriched after treatment (β ≥ +1), blue are depleted after treatment (β ≤ –1), black are insignificant after treatment, genes in the ER pathway are orange, the CDK pathway are purple, and the MTORC1 pathway are green. ( F ) β scores for individual genes depleted after imlunestrant treatment in the ER pathway, ( G ) the CDK pathway, or ( H ) the MTORC1 pathway.
    Figure Legend Snippet: ( A ) Cartoon schematic of the genome-wide CRISPR/Cas9-knockout (KO) screen; (step 1) T47D cells are infected with the H3 lentiviral library of gRNAs, (step 2) infected cells are selected using puromycin, (step 3) cells are collected for untreated baseline (Day 0), or treated with DMSO or 1 nM imlunestrant, (step 4) after treatment for 14 or 31 days cells are collected, gRNAs amplified, and sequenced, followed by (step 5) gRNA analysis using MAGeCK algorithm. ( B ) MAGeCK β score and ranking results for gRNAs enriched or depleted after treatment with DMSO versus Day 0 for 14 days or ( C ) 31 days. ( D ) MAGeCK β score and ranking results for gRNAs enriched or depleted after treatment with imlunestrant versus Day 0 for 14 days or ( E ) 31 days. Scatter plot with each dot represents a gene in the genome-wide gRNA library, red are enriched after treatment (β ≥ +1), blue are depleted after treatment (β ≤ –1), black are insignificant after treatment, genes in the ER pathway are orange, the CDK pathway are purple, and the MTORC1 pathway are green. ( F ) β scores for individual genes depleted after imlunestrant treatment in the ER pathway, ( G ) the CDK pathway, or ( H ) the MTORC1 pathway.

    Techniques Used: Genome Wide, CRISPR, Knock-Out, Infection, Amplification



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    h3 genome wide grna library  (Addgene inc)
    93
    Addgene inc h3 genome wide grna library
    ( A ) Cartoon schematic of the genome-wide CRISPR/Cas9-knockout (KO) screen; (step 1) T47D cells are infected with the <t>H3</t> lentiviral library of gRNAs, (step 2) infected cells are selected using puromycin, (step 3) cells are collected for untreated baseline (Day 0), or treated with DMSO or 1 nM imlunestrant, (step 4) after treatment for 14 or 31 days cells are collected, gRNAs amplified, and sequenced, followed by (step 5) <t>gRNA</t> analysis using MAGeCK algorithm. ( B ) MAGeCK β score and ranking results for gRNAs enriched or depleted after treatment with DMSO versus Day 0 for 14 days or ( C ) 31 days. ( D ) MAGeCK β score and ranking results for gRNAs enriched or depleted after treatment with imlunestrant versus Day 0 for 14 days or ( E ) 31 days. Scatter plot with each dot represents a gene in the genome-wide gRNA library, red are enriched after treatment (β ≥ +1), blue are depleted after treatment (β ≤ –1), black are insignificant after treatment, genes in the ER pathway are orange, the CDK pathway are purple, and the MTORC1 pathway are green. ( F ) β scores for individual genes depleted after imlunestrant treatment in the ER pathway, ( G ) the CDK pathway, or ( H ) the MTORC1 pathway.
    H3 Genome Wide Grna Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/h3+genome+wide+grna+library/pmc12226049-247-7-12?v=Addgene+inc
    Average 93 stars, based on 1 article reviews
    h3 genome wide grna library - by Bioz Stars, 2026-07
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Cartoon schematic of the genome-wide CRISPR/Cas9-knockout (KO) screen; (step 1) T47D cells are infected with the H3 lentiviral library of gRNAs, (step 2) infected cells are selected using puromycin, (step 3) cells are collected for untreated baseline (Day 0), or treated with DMSO or 1 nM imlunestrant, (step 4) after treatment for 14 or 31 days cells are collected, gRNAs amplified, and sequenced, followed by (step 5) gRNA analysis using MAGeCK algorithm. ( B ) MAGeCK β score and ranking results for gRNAs enriched or depleted after treatment with DMSO versus Day 0 for 14 days or ( C ) 31 days. ( D ) MAGeCK β score and ranking results for gRNAs enriched or depleted after treatment with imlunestrant versus Day 0 for 14 days or ( E ) 31 days. Scatter plot with each dot represents a gene in the genome-wide gRNA library, red are enriched after treatment (β ≥ +1), blue are depleted after treatment (β ≤ –1), black are insignificant after treatment, genes in the ER pathway are orange, the CDK pathway are purple, and the MTORC1 pathway are green. ( F ) β scores for individual genes depleted after imlunestrant treatment in the ER pathway, ( G ) the CDK pathway, or ( H ) the MTORC1 pathway.

    Journal: JCI Insight

    Article Title: Imlunestrant a next-generation oral SERD overcomes ESR1 mutant resistance in estrogen receptor–positive breast cancer

    doi: 10.1172/jci.insight.188051

    Figure Lengend Snippet: ( A ) Cartoon schematic of the genome-wide CRISPR/Cas9-knockout (KO) screen; (step 1) T47D cells are infected with the H3 lentiviral library of gRNAs, (step 2) infected cells are selected using puromycin, (step 3) cells are collected for untreated baseline (Day 0), or treated with DMSO or 1 nM imlunestrant, (step 4) after treatment for 14 or 31 days cells are collected, gRNAs amplified, and sequenced, followed by (step 5) gRNA analysis using MAGeCK algorithm. ( B ) MAGeCK β score and ranking results for gRNAs enriched or depleted after treatment with DMSO versus Day 0 for 14 days or ( C ) 31 days. ( D ) MAGeCK β score and ranking results for gRNAs enriched or depleted after treatment with imlunestrant versus Day 0 for 14 days or ( E ) 31 days. Scatter plot with each dot represents a gene in the genome-wide gRNA library, red are enriched after treatment (β ≥ +1), blue are depleted after treatment (β ≤ –1), black are insignificant after treatment, genes in the ER pathway are orange, the CDK pathway are purple, and the MTORC1 pathway are green. ( F ) β scores for individual genes depleted after imlunestrant treatment in the ER pathway, ( G ) the CDK pathway, or ( H ) the MTORC1 pathway.

    Article Snippet: For the CRISPR screen we used the H3 genome wide gRNA library (Addgene #133914) that targets 18,000 annotated genes in the human genome, with 6 gRNAs per gene on average for a total of 117,587 gRNAs and 3,842 control gRNAs targeting AAVS1, ROSA26, and CCR5.

    Techniques: Genome Wide, CRISPR, Knock-Out, Infection, Amplification